Description
The KAPA Long Range PCR system is a blend of Taq DNA polymerase and an engineered archaeal (B-family) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support long-range and/or sensitive PCR, and possess 3 – 4X higher fidelity than Taq polymerase. In the hot-start formulation, KAPA Long Range is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.
KAPA Long Range is also supplied in 2X ReadyMix format with loading dye that contains all the components required for PCR except primers and template. The loading dye in the reaction buffer allows you to load PCR products directly onto the agarose gel with no extra steps.
Kits include KAPA Long Range or KAPA Long Range HotStart DNA Polymerase (5 or 2.5 U/µL), MgCl2-free KAPA Long Range Buffer (5X), high-quality dNTP Mix (10 mM each) and MgCl2 (25 mM). KAPA Long Range HotStart is also available in KAPA Long Range HotStart ReadyMix with dye (2X), which contains DNA Polymerase, reaction buffer with loading dye, dNTPs and MgCl2 (at a final concentration of 2 mM).